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The risk of compromised RT-qPCR results
Features High sensitivity - optimized for two-step quantitative RT-PCR Broad dynamic range Unbiased cDNA synthesis - complete 5’ to 3’ RNA sequence representation Easy reaction assembly
The measured Cq and the shape of the amplification curve reflect inhibition
• TATAA SYBR GrandMaster Mix LowROX (V = 2 · 1250 µl
ACTN3 control DNA – WT (wild type) (1 tubes á 100 µl
TATAA Interplate Calibrator IPC kits:TATAA Interplate Calibrator 250 rxn SYBR® The risk of compromised RT-qPCRVariation Compensation The TATAA Interplate Calibrator (IPC) makes it possible for you to compensate for variations between qPCR runs. For practical reasons many qPCR studies involve the use of samples that are processed in more than a single batch or in which the sample set is extended over time. Even over a short time period, variation between qPCR processing runs is observed due to different baseline subtractions and threshold settings. When to use