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Assay (HPLC) ≥99% Melting range 148
Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins
Tryptone and Yeast are present in the medium in elevated concentrations and provide nitrogen
5 (4 g/l) Assay ≥99%
minus Arginine and without Lysine
SIH Medium Size:250g Assay (HPLC) ≥99% Melting rangeSIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1. 1 mM) and increased levels of Lysine (8. 5 mM) and Tryptophane (1. 7 mM). Concentrations of Cysteine, Glutamic
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